Test procedure

The WeeFoal™ test can be performed on a urine sample collected from
120 days after breeding to day 300. It is not recommended to use the test
before 120 days or after day 300 as results maybe inconclusive. Run the
test at room temperature on a white paper or paper towel in a well-lighted
area. The urine sample and test pack should be at room temperature
before performing the test. The urine sample must be diluted as described
below.

I have found the easiest way to collect urine is to put a mare in a clean stall
with fresh shavings.  Then slip a cup under the tail to catch a small amount
of urine.  

Do not run the test with undiluted urine.

When ready to perform the test, open the foil pouch and place the test
cassette, 2 plastic pipettes and the small tube of urine diluent on a flat,
clean surface. Take one of the pipettes, squeeze the upper bulb and draw
enough of the urine sample into the pipette to fill the stem completely. (0.15
ml will fill the stem, and any overdrawn amount will go into the lower bulb
reservoir). Discharge the urine sample from the pipette stem into the small
tube of urine diluent by gently squeezing the upper bulb (any excess fluid
from the draw will remain in the reservoir). Replace the cap on the small
tube of urine diluent and invert the tube twice to thoroughly mix the urine with
the diluent. Take the second pipette, put the stem in the tube of diluted
urine, and draw enough of the diluted urine sample into the pipette to fill the
stem completely. Discharge the diluted urine sample drop by drop from the
pipette stem into the round sample well of the cassette by gently squeezing
the upper bulb (any excess fluid from the draw will remain in the reservoir).










Allow the sample to soak into the absorbent pad in the well between drops.
Once a few drops have been added to the sample well, the urine will start to
flow along the membrane in the cassette window. Continue adding drops of
diluted urine until the leading edge of the fluid is ¾ of the way along the
membrane, i.e. has reached the ‘C’ mark on the plastic housing.
Do not flood the sample well by adding too much sample, or adding it too
quickly.
Wait for 5 to 10 minutes. The test result will generally be obvious within 5
minutes but occasionally it may take up to 10 minutes for the result to
clearly form.
Positive               Negative
Interpreting the result
WeeFoal™ Equine Urine Tests
Works on Horses, Zebras &
Donkeys